Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability

It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast. However, we show that {Delta}mto1 cells, which are defective in nucleation of cytoplasmic microtubules, have profoundly misoriented spindles but are not delayed in the timing of sister chromatid separation, providing compelling evidence that fission yeast does not possess a spindle orientation checkpoint. Instead, we show that latrunculin A delays anaphase onset by disrupting interpolar microtubule stability. This effect is abolished in a latrunculin A-insensitive actin mutant and exacerbated in cells lacking Ase1, which cross-links antiparallel interpolar microtubules at the spindle midzone both before and after anaphase. These data indicate that both Ase1 and an intact actin cytoskeleton are required for preanaphase spindle stability. Finally, we show that loss of Ase1 activates a checkpoint that requires only the Mad3, Bub1, and Mph1, but not Mad1, Mad2, or Bub3 checkpoint proteins

A Study of Optical Observing Techniques for Extra-Galactic Supernova Remnants: Case of NGC 300

We present the results of a study of observational and identification techniques used for surveys and spectroscopy of candidate supernova remnants (SNRs) in the Sculptor Group galaxy NGC 300. The goal of this study was to investigate the reliability of using [Sii]/Halpha > 0.4 in optical SNR surveys and spectra as an identifying feature of extra-galactic SNRs (egSNRs) and also to investigate the effectiveness of the observing techniques (which are hampered by seeing conditions and telescope pointing errors) using this criterion in egSNR surveys and spectrographs. This study is based on original observations of these objects and archival data obtained from the Hubble Space Telescope which contained images of some of the candidate SNRs in NGC 300. We found that the reliability of spectral techniques may be questionable and very high-resolution images may be needed to confirm a valid identification of some egSNRs.Comment: 27 Figures, 10 table

Is voice therapy an effective treatment for dysphonia? A randomised controlled trial

OBJECTIVES: To assess the overall efficacy of voice therapy for dysphonia. DESIGN: Single blind randomised controlled trial. SETTING: Outpatient clinic in a teaching hospital. Participants: 204 outpatients aged 17-87 with a primary symptom of persistent hoarseness for at least two months. INTERVENTIONS: After baseline assessments, patients were randomised to six weeks of either voice therapy or no treatment. Assessments were repeated at six weeks on the 145 (71%) patients who continued to this stage and at 12-14 weeks on the 133 (65%) patients who completed the study. The assessments at the three time points for the 70 patients who completed treatment and the 63 patients in the group given no treatment were compared. MAIN OUTCOME MEASURES: Ratings of laryngeal features, Buffalo voice profile, amplitude and pitch perturbation, voice profile questionnaire, hospital anxiety and depression scale, clinical interview schedule, SF-36. RESULTS: Voice therapy improved voice quality as assessed by rating by patients (P=0.001) and rating by observer (P<0.001). The treatment effects for these two outcomes were 4.1 (95% confidence interval 1.7 to 6.6) points and 0.82 (0.50 to 1.13) points. Amplitude perturbation showed improvement at six weeks (P=0.005) but not on completion of the study. Patients with dysphonia had appreciable psychological distress and lower quality of life than controls, but voice therapy had no significant impact on either of these variables. CONCLUSION: Voice therapy is effective in improving voice quality as assessed by self rated and observer rated methods

Accurate simulations of the interplay between process and statistical variability for nanoscale FinFET-based SRAM cell stability

In this paper we illustrate how by using advanced atomistic TCAD tools the interplay between long-range process variation and short-range statistical variability in FinFETs can be accurately modelled and simulated for the purposes of Design-Technology Co-Optimization (DTCO). The proposed statistical simulation and compact modelling methodology is demonstrated via a comprehensive evaluation of the impact of FinFET variability on SRAM cell stability

Business strategy and the environment Tesco Plc's declining financial performance and underlying issues

The case presents a teaching tool which requires students to: 1) analyze the financial performance of Tesco Plc over the last four years; 2) compare Tesco’s market position with key competitors; 3) identify and evaluate Tesco’s business strategy; 4) evaluate the causes of Tesco’s decline in performance; 5) develop recommendations to address declining performance; 6) identify and evaluate the Human Resource strategic role in addressing and supporting performance. The case is suitable for a business strategy or human resource strategy class. The case is appropriate for use at the undergraduate or masters level. Students should have some familiarity with business and human resource management strategy before being assigned to the case. Students might be assigned to work individually or in teams on the project. Individuals or groups may be required to present their research to the class for discussion and comment. Six to ten hours outside of class should be required to complete the case study exercise. Classroom discussion should be between two to three hours

Réplica a Maximiliano Salinas

En relación con la crítica que Maximialiano Salinas hace de mi libro Misticismo e Inquisición en el virreinato peruano. Los procesos a los alumbrados de Santiago de Chile 1710-1736, debo indicar que ella deja en evidencia una actitud bastante especial de parte de su autor. En el fondo, hace caso omiso de la tesis central del libro y se limita a señalar algunas obras que se habrían omitido y a reiterar su propia interpretación del fenómeno estudiado, que había expuesto de manera sucinta hace algún tiempo

Talent management and developing leadership talent

An important aspect of talent management in the contemporary organization is the investment and development of leaders including leadership development. Developing leadership talent is viewed as a key lever in delivering competitive advantage in business today. Leadership can be viewed from multiple perspectives: as a personality with special traits or characteristics; an act or behavior shown by the leader; the capability, knowledge, and skills demonstrated by the leader; the process used by the leader to bring groups together; or transformative change and the power relationship that exists between leaders and followers. In essence, developing leadership talent is a multi-layered process, ideally involving a blended learning approach that incorporates formal, informal, and experiential learning. The development of the leader as an individual focuses on building and enhancing the individual's capability to undertake the role of a leader. A key aspect of leader development is that “it is ultimately about facilitating an identity transition” to create new leadership options

Supporting security-oriented, collaborative nanoCMOS electronics research

Grid technologies support collaborative e-Research typified by multiple institutions and resources seamlessly shared to tackle common research problems. The rules for collaboration and resource sharing are commonly achieved through establishment and management of virtual organizations (VOs) where policies on access and usage of resources by collaborators are defined and enforced by sites involved in the collaboration. The expression and enforcement of these rules is made through access control systems where roles/privileges are defined and associated with individuals as digitally signed attribute certificates which collaborating sites then use to authorize access to resources. Key to this approach is that the roles are assigned to the right individuals in the VO; the attribute certificates are only presented to the appropriate resources in the VO; it is transparent to the end user researchers, and finally that it is manageable for resource providers and administrators in the collaboration. In this paper, we present a security model and implementation improving the overall usability and security of resources used in Grid-based e-Research collaborations through exploitation of the Internet2 Shibboleth technology. This is explored in the context of a major new security focused project at the National e-Science Centre (NeSC) at the University of Glasgow in the nanoCMOS electronics domain

Study of volatile contaminants in reclaimed water

Different methods were evaluated for reducing the volatile contaminants found in water recovered from urine by distillation. The use of activated carbon, addition of potassium permanganate, and the use of oxidation catalyst are described along with laboratory tests. It is concluded that catalytic decomposition appears to be feasible, and further investigation is recommended